Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly used cell culture model? In this blog we will spill the beans on all things HEK, from the first experiments isolating the cell line all the way to modern applications.
Viruses are the SUVs of molecular biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a variety of terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...
I was excited to see that Addgene has recently added a new feature for depositing labs to its website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is a rich source of information and has data on the date, material, requesting PI ...
If you are lucky in lab life, you will have a plethora of antibody options for your experiment (all well validated for your application, of course!) When the stars are aligned and the lab gods are smiling down at you, you may wonder which antibody should I pick? Do I go for the ...
We all know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene. But what about the other yeasts – in the biomedical ...
Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and off by binding proximal to their target genes. Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression (and most everything ...
Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.
.png)
-min.jpg)
