Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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I was excited to see that Addgene has recently added a new feature for depositing labs to its website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is a rich source of information and has data on the date, material, requesting PI ...
If you are lucky in lab life, you will have a plethora of antibody options for your experiment (all well validated for your application, of course!) When the stars are aligned and the lab gods are smiling down at you, you may wonder which antibody should I pick? Do I go for the ...
We all know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene. But what about the other yeasts – in the biomedical ...
Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and off by binding proximal to their target genes. Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression (and most everything ...
Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.
This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make a plasmid? Or how much time you have to spend cloning before you can start your experiment? What about all the reagents you need to order? Sometimes, ...
Viruses have many negative associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows them to easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But ...
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