Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst. While it is tempting to just dive right in and start injecting your virus, a successful AAV experiment starts with validation and optimization. Although there are different ...
Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. AAVs are non-pathogenic, do not integrate into the host genome, and can be ...
Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for data pipeline processing (Nextflow), cloud infrastructure, and secure collaboration.
You may be familiar with immunofluorescence (IF, often referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds a target protein in your sample, then a fluorescently labeled secondary antibody ...
When it comes to using antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc, domain. Sure, we all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies to bind. We also ...
The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that works for your application, specifically detects your protein, is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the ...
The biomedical field is often concerned with understanding the cause of diseases and how to treat those diseases. The “cause of disease” often requires understanding the disease genetics and the “treatment” usually requires drugs. While we often think of these two fields as ...
