As the saying goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do a few calculations. This calculator can do the math for you and it’s simple to make adjustments for the type of tissue culture dish and number of dishes used. Here’s to making AAV packaging a little easier!
Topics: Viral Vectors
Plasmids can be amazing and simple tools for studying gene regulation. They are used to study how transcription factors and other trans-regulatory elements (TREs) and some cis-regulatory elements (CREs), like promoters, influence gene expression. However, scientists frequently return to native chromosomes because chromatin context matters. The impact of TREs and CREs on gene expression is commonly investigated via Chromatin Immunoprecipitation (ChIP) and chromatin capture techniques, respectively, but these two separate methods are not without their own technical challenges. Enter the Xu Lab's CAPTURE, a method for identifying TREs and CREs that partners CRISPR’s targeting abilities with the strength of the biotin-streptavidin interaction. CAPTURE is capable of identifying old and new TREs and CREs, CRE-CRE interactions, and has even provided enough data for Liu et al to re-draw the beta-globin locus regulation model. Read on to learn more about this captivating tool!
In this post of the Careers in Science Communication blog series, you’ll hear more about Caitlin Runne-Janczy and her job as a Subject Matter Expert/ Product Development Manager at eScience Labs. To learn how Caitlin got into scicomm and landed her first post-grad school job, head over to part I of the interview.
In this post in the Careers in Science Communication blog series, you’ll learn about Caitlin Runne-Janczy, a Product Development Manager at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum to support them. Caitlin’s interview is broken into two parts, with part one detailing how she got into scicomm and part two focusing on what her job at eScience Labs is like. Find all the posts in this series here.