By Rachel Leeson
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I was excited to see that Addgene has recently added a new feature for depositing labs to its website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is a rich source of information and has data on the date, material, requesting PI ...
Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and off by binding proximal to their target genes. Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression (and most everything ...
This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make a plasmid? Or how much time you have to spend cloning before you can start your experiment? What about all the reagents you need to order? Sometimes, ...
Turn it on? Turn it off? Turn it down, but not forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression control tools as well as ...
What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used to study gene expression in cells. But these reporters have limited use in vivo because they are based on light which is very easily scattered by tissue. Ultrasound, on the ...
When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut DNA control: a band or two appear on an agarose gel at notably higher molecular weights than expected, given the size of the plasmid. This is seen only in the uncut DNA; ...
Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many ...
