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Modulate the Activity of 17 Signaling Pathways with One Kit!

Posted by Kendall Morgan on Mar 22, 2016 10:30:00 AM

When cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable but fleeting.

“What’s been shown by others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center. “While we do not yet have a comprehensive understanding of how cancers become resistant, an emerging theme is that they do so by activating signaling pathways controlling properties like growth, survival, and differentiation.”

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Topics: Plasmid Technology, Cancer, Plasmid Kits

CRISPR 101: Multiplex expression of gRNAs

Posted by Mary Gearing on Jan 28, 2016 10:50:00 AM

This post was updated on Dec 5, 2017.

CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple gRNAs. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian plasmids to introduce you to some of your potential options and cloning methods, but please scroll down for plasmids suitable for other model systems, including E. coli, plants, Drosophila, and zebrafish!

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Topics: Genome Editing, CRISPR, CRISPR 101, Plasmid Kits

28 Hot Plasmid Technologies from 2015

Posted by Tyler Ford on Dec 23, 2015 10:30:00 AM



At Addgene we're continually impressed with the amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all of them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it!

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Topics: Plasmid Technology, Hot Plasmids, Plasmid Kits

Easing the Protein Purification Process with pCri

Posted by Mary Gearing on Jun 19, 2015 11:08:00 AM

Protein purification can be one of the most stressful lab activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein, but getting to this stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed from a plasmid construct, most often in special E. coli strains designed for protein expression. Cultures are then lysed and the protein of interest is purified using an affinity tag. Additional tags may be used to improve protein stability and solubility.

Determining the best way to express one’s protein of interest can save a lot of time later.

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Topics: Hot Plasmids, Plasmid Kits

22 Hot Plasmid Technologies from 2014

Posted by Joanne Kamens on Jan 6, 2015 12:21:53 PM

Updated Mini-transposon Vector for Bacterial Mutagenesis or Gene Targeting

Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or other markers into a target genome. They did this by selecting the important elements from existing transposon and vector systems and creating an all-synthetic vector that included only the elements needed for function.

The lab validated this vector, called pBAM1, by conducting random mutagenesis in the soil bacterium Pseudomonas putida and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the genome. Although this tool was published in 2011, it was only recently made available through Addgene and we want to highlight it for use in your research.

Check out Joanne's Reddit AMA

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Topics: Hot Plasmids, Lab Tips, Plasmid Kits

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