Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Fluorescent proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression, localization, and protein-protein interactions. Beyond these common applications, researchers ...
Signal transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to the next members of their pathways, but where the kinases are located, their signal strength, and how long their signals last all impact transduction of the message. To study ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene (YFG) in flies. Read the first post here. Once ...
We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can improve your heart health. These are amazing advances that will surely save thousands if not millions of lives, but many throughout the world are affected by devastating ...
Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing countries. We are proud to introduce the 5 ...
Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. Do you have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in the Drosophila ...
