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Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over the last two decades, but their use can be limited by a few caveats. Since fluorescent proteins (FP) require external light activation, you can’t use fluorescence to monitor ...
Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are biological tools that monitor a process or detect a given molecule. The sensor component is usually a protein that undergoes a conformational change in response to the ...
We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new ...
Have you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy tool for you.
This post was contributed by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies to map the mutations ...
Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. For a few reasons, Cpf1 is a simplified system for editing multiple targets compared to Cas9. ...
Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might dye your cells and look at them under a microscope, fractionate samples to isolate particular organelles and ...
