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Rosella is a pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating”) is induced by a lack of ...
What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting and amplifying RNA sequences that could bind very specifically to target molecules. Using a technique called systematic evolution of ligands by ...
Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute for Cell Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge ...
This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’re like many researchers these days, you are ready to take (if you haven’t already) the plunge into the world of precision genome editing. When it comes time to (hopefully) ...
In biology as in life, more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology, the Vale and ...
In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral ...
If you are interested in using lentiviral vectors to introduce your favourite gene into your favourite cell line or into primary cells, this blog article will give you some tips to plan your experiment using the lentiviral vector system. Viral vectors have been increasingly ...
