Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the years since, our scientists have optimized many steps in the viral production and quality control process to improve both the yield and ...
When I started writing for the Addgene blog, I was focused on writing about new scientific techniques and cool plasmids. Creating graphics were usually the last thing I thought about when writing posts. Since then I’ve realized my figures are just as important, if not more ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
The late Michael Davidson and Roger Tsien played enormous roles in fluorescent protein development by openly sharing their resources with the research community. To honor their legacy, we launched the Michael Davidson and Roger Tsien Commemorative Travel Awards in 2017. These ...
While much of CRISPR research has focused on genome editing, numerous discoveries have been made using the Cas9 nuclease in the absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as dCas9 (Jinek et al., 2012). Like Cas9, dCas9 can ...
In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research! This ...
So you’ve done the research, gathered up your data into an exciting story, and are ready to present your findings at a conference. But what you get out of a conference depends on what you put into it before, during, and after the meeting. Let’s break it down into the following: ...
