Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Direct reprogramming describes the process where differentiated cells are turned into a cell type of choice while bypassing the intermediate pluripotent state. Though a valuable tool for regenerative medicine, direct reprogramming is an inefficient process, with the majority of ...
Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized. This represents a considerable barrier to furthering research and developing effective treatments for the disease. Recently, Kenneth Scott’s lab at Baylor College of Medicine ...
In this heyday of molecular biology, many scientists do a lot of DNA work but never get to actually manipulate the organism they study (unless if you count normal human interaction for all of us studying human genes in test tubes and gels). As a freshman in college I studied ...
We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen. This fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at ...
This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy but effective habits you can incorporate to reduce your lab consumption and increase your lab sustainability. For my other posts on lab sustainability, check out my Recycling ...
The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition, among others. Over the past few years, dCas9 ...
This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 with the goal of communicating science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto, created The ...
