Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet alone study.
Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal networks, and specific neuronal populations to consider. To tease apart how different neurons impact a certain output, ...
In 2016, I was put in charge of the biggest project I’d seen in my already long tenure at Addgene. I would work with our research team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new viral services. After a month or so of ...
It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: Option #1: Affinity tag purification You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several ...
In 2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – large tracts of the microbial tree of life that remained unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic (pure) ...
This post was contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone who works in a lab struggles with reproducibility at some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce someone else’s ...
In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most recent ...
