Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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In 2016, I was put in charge of the biggest project I’d seen in my already long tenure at Addgene. I would work with our research team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new viral services. After a month or so of ...
It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: Option #1: Affinity tag purification You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several ...
In 2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – large tracts of the microbial tree of life that remained unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic (pure) ...
This post was contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone who works in a lab struggles with reproducibility at some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce someone else’s ...
In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most recent ...
Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween is no exception. For the past 10+ years, Addgenies have formed teams, chosen a theme for the costume, ...
Flash quiz! What pops into your head when you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. However, ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ...
