Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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We first “went viral” in 2016 when we introduced the viral service, and now we’re “going viral” again!
Part stable scaffold, part epitope binding region, all fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements of an antibody probe, scientists can now more easily ...
Dear scientists using AAV, We hear your questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should I use to target my favorite neurons? We don’t know all of the answers, but we’re betting that your colleagues do. That’s why we’re ...
From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene. Since it’s National Book Month, we’ve asked some Addgenies to share their favorite books - both scientific and not - to inspire you to read a little more this October.
To LinkedIn or not to LinkedIn. That is the question. When presenting on building relationships (also known as “networking”), one of the most common questions I receive is “Do I have to be on LinkedIn?” For anyone who is planning or might need to plan for a career outside ...
CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction; but these methods aren’t stealthy enough to gain ...
This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR could fail to produce a product, the transformation may not result in any ...
