Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. You just need three ...
Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research labs closed during the pandemic, it’s hard to get these reagents. However, when reagents have been made available through a repository, they remain accessible during these ...
Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for ...
Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very first subheading in Chapter 1 asks “What is science?” That’s a very big question. Zimmer, a Professor of Chemistry at Connecticut ...
Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing, activating gene expression, repressing gene expression, and much more. But there’s one thing ...
You’ve prepped your DNA and you’re ready to get started on the next step of your experiment. But in many cases, you won’t see any signs of DNA in your final tube after purification. How do you know if you actually have DNA in your tube without seeing it? There are many ways to ...
This post was contributed by Max W. Shen from MIT, Alvin Hsu Harvard University, and David R. Liu from the Broad Institute and Harvard University. Over the course of the last six months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused ...
