Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for ...
Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very first subheading in Chapter 1 asks “What is science?” That’s a very big question. Zimmer, a Professor of Chemistry at Connecticut ...
What if after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation? Great news. A subset of our ...
Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing, activating gene expression, repressing gene expression, and much more. But there’s one thing ...
You’ve prepped your DNA and you’re ready to get started on the next step of your experiment. But in many cases, you won’t see any signs of DNA in your final tube after purification. How do you know if you actually have DNA in your tube without seeing it? There are many ways to ...
This post was contributed by Max W. Shen from MIT, Alvin Hsu Harvard University, and David R. Liu from the Broad Institute and Harvard University. Over the course of the last six months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused ...
Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity and are excited for this next step. But wait, now you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready for this ...
