RNA Extraction Without A Kit

By Leah Schwiesow

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Workflow of phage-assisted evolution of base-editing activity. Fresh host cells contain Cas, mutated gIII, and mutagenesis plasmids. Phage infection delivers the deaminase plasmid. After mutagenesis, if the base editor is inactive, no pIII is produced, and the progeny phage are not infectious. If the base editor is active, however, pIII is produced and the progeny phage are infectious, allowing them to continue propagating.
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