Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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My name is Lauren Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the plasmids that go out our doors are correct.
Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence. Dysregulation of the epigenome (the sum of epigenetic modifications across the genome) has been ...
By Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share their reagents, we knew our role in fighting the pandemic could be even more impactful by reaching more scientists. Many members of the scientific community support open ...
Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the ...
Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important to consider your experiment. It will dictate the amount of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation in 3 ...
Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While they serve similar purposes, both techniques offer researchers different advantages and limitations. The four main factors in which ...
If you use a kit for DNA purification or if you use a DIY purification protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does ...
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