Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

By Kendall Morgan

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Multiplexed insertion is possible using a single plasmid that contains a multi-spacer CRISPR array. This encodes separate gRNAs that target different locations in the genome.
The RepCap plasmid consists of three promoters within the rep coding region that encodes four different Rep proteins. This region also encodes three different VP proteins, the MAAP protein, and the AAP protein.
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Schematic of the human MECP2 locus and MECP2 protein.
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The Origin of Replication

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