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Immunoprecipitation (IP) uses immobilized antibodies, or immunoglobulins, to isolate a specific protein out of a complex mix. Using this technique, users can look for the presence or absence of a protein, determine if a protein is up or downregulated, examine a protein’s ...
It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures, our first of the season snow flurries, dusting off the winter coats and hats, and our annual Deck The Lab holiday decorating contest!
A 2017 survey found that many researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering advice on ...
It’s time for another edition of “What’s New in CRISPR” - where we highlight a few of the newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content. In this post we will cover: Miniature ...
Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions like: “Where does my protein of interest live within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact ...
Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful ...
There are a lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the role it plays in an immune response. But ...
