Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists. What works well in one cell or neuron type may not work well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression ...
A few months ago, we shared an introduction to immunofluorescence (IF) - a common method for visualizing molecules of interest within a cell or tissue. In that introduction, we broke down the method into six general steps and outlined the considerations to be made during each ...
I’ve always been fascinated by the human body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all the optional sexual health sessions and reported the intel back to my ...
As a scientist, perhaps the most important part of your research is communicating the results of the work. Yes, those control experiments are important — but without letting other scientists know the results, your work won’t have any impact!
Have you ever been stuck googling about an antibody that will be essential to your new research project, but unsure which one will mark your target the best, or even which one will work for your application? Like many scientists, I have spent hours sifting through comments and ...
In a lab, you may have heard the phrase gram negative or positive being used to describe a species of bacteria, but what does it actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the ins ...
I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished my undergraduate degree (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience major, I was struck with the realisation that I didn’t want ...
