Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Have you ever been stuck googling about an antibody that will be essential to your new research project, but unsure which one will mark your target the best, or even which one will work for your application? Like many scientists, I have spent hours sifting through comments and ...
In a lab, you may have heard the phrase gram negative or positive being used to describe a species of bacteria, but what does it actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the ins ...
I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished my undergraduate degree (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience major, I was struck with the realisation that I didn’t want ...
From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing laboratories and associated publications, but our quality control (QC) ...
The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety of ...
If you're interested in sharing your science, one of the big questions you'll be faced with is "how?" How are you going convey your information to your audience? There's so many ways options available to today's science communications: podcasts, blogging, videos, lectures, ...
This post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University. There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility ...
