Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your deposit. This is because repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid diversity, so ...
When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV) light, they do so via ...
Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.
If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter. After all, they’re less common than they were ten years ago, and it does take a bit of time to craft one. We still think they're useful, so we've updated our cover letter ...
In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to another. When you label your samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission spectra, meaning they will emit fluorescent ...
Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free expression systems, mammalian cells, yeast, flies, and even plants! In this blog we will review common ...
The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise awareness of the economic and cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids too, though not just for ...
