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When using flow cytometry to analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure your cells of interest. In many experiments you’ll be working with a heterogeneous cell population, for example from a processed piece of ...
Integrins are essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and extracellular environments. Found on nearly every cell in the human body, integrins are key to a diverse array of processes, including cell adhesion, migration, ...
While the antibodies present throughout our bodies carry out plenty of roles just the way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly because, well, antibodies are kind of hard to see. To solve this issue, researchers ...
Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and cell cultures, opening biological interiors to many inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical processes that are crucial in ...
Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE isotype?"
In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and exogenously expressed proteins. Here we will review what advantages degron tags can offer over other protein control methods and compare the commonly available types ...
As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you have been in the cloning trenches yourself. Modular cloning, or MoClo, is a systematic cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning ...
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