Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral ...
An enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to measure antibody levels, scientists have since adapted it for a host of different proteins ...
If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer agreement (MTA) process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a mystery for those who aren’t familiar with it. If ...
There are several facilities nationwide where you can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in your own lab with standard molecular biology tools and tissue culture experience. Here, we’ll go over the basics of rAAV ...
We are very excited to share the latest edition of our Viral Vectors 101 eBook!
What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20 additions we've made to our viral vectors repository since March.
CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but ...
-min.png)
-min.png)