Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through how we produce both our AAV preps and our lentiviral preps.
Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among the most important are the inverted terminal repeats (ITRs). ITRs are the ...
The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally, delivery is achieved through the use of ...
Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there too!). Calcium is fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural component of bones and teeth. It is an enzyme ...
The Retroviridae (commonly called retrovirus) family — of which HIV is a member — may seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses” is an umbrella term ...
In order to understand the complex wiring of the brain and the underpinnings of neurological disease, neuroscientists need to be able to probe cells and circuits without disrupting normal brain function. Chemogenetics has become a popular tool in the field as it provides a ...
The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions; you can quantify them with biosensors! In this blog we will review the basics of biosensors ...
In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to blue light, this channel activates neuronal ...
