AAV Q&A with Tim Miles

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Schematic of Plasmid Amplification in Recombinase Deficient e. Coli
Electron micrograph showing hM4Di tagged with mCherry remaining cytoplasmic and hM4Di-HA tag localizes to the plasma membrane.
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The RepCap plasmid consists of three promoters within the rep coding region that encodes four different Rep proteins. This region also encodes three different VP proteins, the MAAP protein, and the AAP protein.
Graphic of channelrhodopsin activated with blue light. Without light, the channelrhodopsin is inactive, no ions flow in, and neurons don't fire. With blue light, the channelrhodopsin opens and allows an influx of ions into the neuron. This results in neuronal firing.

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