The 12 Days of CRISPR: 2021

By Rachel Leeson

One of our favorite holiday traditions is our annual Twelve Days of CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources, fear not! You can find all of them here. 

On the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use.

On the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency.

On the third day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND).

On the fourth day of CRISPR, Addgene gave to me: enhanced prime editing systems through manipulating MMR machinery.

On the fifth day of CRISPR, Addgene gave to me: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs.

On the sixth day of CRISPR, Addgene gave to me: PRIME-del (Precise genomic deletions). 

On the seventh day of CRISPR, Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease.

On the eighth day of CRISPR, Addgene gave to me: the PEDAR strategy to delete and replace long genomic sequences.

On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration. 

On the tenth day of CRISPR, Addgene gave to me: a primer on prime editing

On the eleventh day of CRISPR, Addgene gave to me: a Cas9 nickase 101 blog post

On the twelfth day of CRISPR, Addgene gave to me: an updated CRISPR 101 e-book (to help with the other 11 presents!) 

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Topics: CRISPR

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