By Beth Kenkel
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So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. You just need three ...
One of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving the exact materials that they think they are. To address this, several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS) QC ...
Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure your gene goes where you want it to? Knowing that a gene is expressed in a particular cell type is important not only for translational research, such as gene therapy, but ...
Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often repeat this assay to confirm that our results are accurate. But what if we could repeat this assay 10,000 times to ensure that our titers ...
Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal networks, and specific neuronal populations to consider. To tease apart how different neurons impact a certain output, ...
One of the biggest barriers to gene therapy treatment of central nervous system (CNS) diseases is the blood brain barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV), from reaching ...
Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the years since, our scientists have optimized many steps in the viral production and quality control process to improve both the yield and ...
