Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst. While it is tempting to just dive right in and start injecting your virus, a successful AAV experiment starts with validation and optimization. Although there are different ...
Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. AAVs are non-pathogenic, do not integrate into the host genome, and can be ...
We often think about the brain’s function in terms of its regions. But equally important is the way the brain connects across these regions, a process driven by neurons. By studying how neurons are physically connected, we can begin to understand how the brain works. It’s kind ...
We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share their AAV validation data. The Data Hub contains a curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...
We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working with AAV, support open science, and maybe even win a $500 travel prize in our open data extravaganza!
Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but he kindly took some time to address all your unanswered questions via text! (well, maybe not all of ...
This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal in 2022. Recent updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins of the system and explore ...
Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful ...
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