Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
This post was contributed by Kusumika (Kushi) Mukherjee, editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. Stem cells are special types of cells that can develop or “differentiate” into more specialized cells with specific functions [1]. In many ...
This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation. There are many motivations to generate transgenic Arabidopsis, from studying ...
My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great co-workers ...
This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Plasmid cloning is an essential part of any molecular biology project, yet very often, it is also a bottleneck in the experimental process. The majority of current ...
This post was contributed by guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly limitations Embraced by the synthetic biology community, Golden Gate Assembly is commonly used to assemble 2–10 DNA fragments in a single ...
This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited job ...
CRISPR pooled libraries have allowed scientists to easily perform genome-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to knock out, inhibit or activate target genes by combining specific sgRNAs with Cas9 or Cas9 derivatives.
