Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Biotin and its binding partner avidin are commonly used today in molecular biology for an array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation, the discovery of the biotin-avidin interaction and the uses of ...
Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca’s Waltham site after doing a brief academic postdoc. Why did I ...
For the last 14 years, scientists have been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository a ...
This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative medicine is to replace lost or damaged cells. Here, I will discuss the two major processes by which an adult somatic cell is converted to a different cell type for ...
Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances toxic to themselves? What are toxin-antitoxin systems? These toxic substances are part of toxin-antitoxin systems ...
This post was contributed by Kusumika (Kushi) Mukherjee, editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. Stem cells are special types of cells that can develop or “differentiate” into more specialized cells with specific functions [1]. In many ...
This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation. There are many motivations to generate transgenic Arabidopsis, from studying ...
