Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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A similar genetic code is used by most organisms on Earth, but different organisms have different preferences for the codons they use to encode specific amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. There are therefore 64 ...
Can you imagine how much space a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone by ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering how many plasmids leave ...
This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics Education Project (pgEd). Advances in genetic technologies and other biomedical innovations promise an improved understanding of how our bodies work, new treatments for ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. These screens provide an alternative to genome-wide RNAi screens, which although highly effective, are affected by low on-target efficacy, ...
CRISPR has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly being developed. Performing a CRISPR experiment requires delivery of, at minimum, two components: the Cas9 protein ...
In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an open-access journal that enables high school students to publish peer-reviewed scientific research. You’ll meet some of the folks behind the journal and hear from a fantastic ...
