Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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CRISPR pooled libraries have allowed scientists to easily perform genome-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to knock out, inhibit or activate target genes by combining specific sgRNAs with Cas9 or Cas9 derivatives.
Why is choosing the right lab such a big deal? It’s actually something you CAN choose and it will make a huge difference for your future career and life. You might see a lab head as choosing you, but in reality, you are giving your hard work and talent for many years (at a very ...
A similar genetic code is used by most organisms on Earth, but different organisms have different preferences for the codons they use to encode specific amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. There are therefore 64 ...
Can you imagine how much space a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone by ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering how many plasmids leave ...
This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics Education Project (pgEd). Advances in genetic technologies and other biomedical innovations promise an improved understanding of how our bodies work, new treatments for ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. These screens provide an alternative to genome-wide RNAi screens, which although highly effective, are affected by low on-target efficacy, ...
