Note: Cpf1 is also called Cas12a.
There’s a new development for CRISPR-Cpf1 genome editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. For a few reasons, Cpf1 is a simplified system for editing multiple targets compared to Cas9. Read on to learn more about Cpf1 multiplexing. For an in-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9. For a brief comparison of Cpf1 vs. Cas9, see the table below.