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Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Posted by Tyler Ford on Dec 16, 2015 10:30:00 AM

As evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on research and will be affecting our everyday lives very soon. Not only is CRISPR having effects on various biological disciplines, the base technology itself is constantly improving. Cas9 variants have been modified for genome editing, activating gene expression, visualizing genomic loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target specificity of the Cas9 nuclease with engineered variants: eSpCas9SpCas9-HF1, & HypaCas9.

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Topics: Genome Engineering, CRISPR

A Better Way to Get Customer Support: The Help Center

Posted by Bryan Archambault on Dec 10, 2015 10:30:00 AM

Addgene’s Customer Service Philosophy

Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When you finally speak to a human being, you may not even get a solution to the problem you called about. Next, you try an email, given that this is the 21st century and all. But while the auto-reply telling you your message was received is a nice touch, it doesn’t help much if the actual response takes 3 days and still doesn’t even answer your question.

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Topics: Inside Addgene

An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

Posted by Tyler Ford on Dec 8, 2015 10:30:00 AM

We recently sat down with Addgene Board Member, depositor, and Harvard Medical School researcher Connie Cepko. Listen to the podcast below to hear all about the research being conducted in Professor Cepko's lab and to get some insight into her management and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing GFP-activated proteins, read our recent blog post.

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Topics: Interview, Viral Vectors, Podcast

Addgene's Top 10 Blog Posts of 2015

Posted by Tyler Ford on Dec 7, 2015 1:00:00 PM

2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent to the research community, two eBooks (Management for Scientists and the Plasmids 101 2nd ed), and have started providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth with our readership expanding from ~10,000 users per month in November 2014 to ~30,000 users per month in November 2015.

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Topics: Fun

Plasmids 101: Stringent Regulation of Replication

Posted by Jason Niehaus on Dec 3, 2015 10:30:00 AM

Plasmids utilize their host cell's replication machinery in order to replicate. As described in our previous Origin of Replication post, DNA replication is initiated at the ORI and may be synchronized with the replication of the host cell's chromosomal DNA or may be independent of the host's cell cycle. 

Plasmids are said to be under stringent control of replication when they are dependent on the presence of initiation proteins synthesized by the host cell in order to start their own replication. In general, these types of plasmids tend to be low copy number. Conversely, plasmids that can initiate DNA replication independently of the host's initiation proteins are said to be under relaxed control, as they only require the host's replication machinery for elongation and termination. These types of plasmids tend to be high copy number.

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Topics: Plasmid Elements, Plasmids 101

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