Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Last updated Apr 17, 2020. Before being adapted by scientists to edit the genome of virtually any organisms on this planet, CRISPR-Cas systems were merely adaptive immune systems that provide bacteria protection against infectious agents. Several enzymes behind this immunity ...
By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers. In the ...
Have you ever found yourself frustrated reading through a paper to find that there is insufficient information about which reagents were used? Unambiguous identification of a reagent is crucial for reproducibility because mistakes in this can lead to wasted time or retractions. ...
Biotin and its binding partner avidin are commonly used today in molecular biology for an array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation, the discovery of the biotin-avidin interaction and the uses of ...
Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca’s Waltham site after doing a brief academic postdoc. Why did I ...
For the last 14 years, scientists have been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository a ...
This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative medicine is to replace lost or damaged cells. Here, I will discuss the two major processes by which an adult somatic cell is converted to a different cell type for ...
