Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Updated April 21, 2020 Picture this: you’re a busy scientist anxiously awaiting the arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last additional experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps you’re about to present your first scientific poster, but unsure how best to prepare. Maybe you’re a presentation veteran, but have difficulty answering questions. Or perhaps ...
With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the beginning of September all will be powered by 100% renewable electricity!
You’ve worked hard to purify your gene of interest, get it into your plasmid backbone, and zap the mixture of DNA into cells. Unfortunately, not every cell successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest, but ...
The aim of behavioral neuroscience is to define brain areas that are linked to a certain behavior. It is a very old branch of science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body problem). Because of the availability of ...
This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations in bacteria, so many that it may be difficult to decide which path to take. A quick and easy way to introduce a mutation in the ...
