Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability to infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction; but these methods aren’t stealthy enough to gain ...
This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR could fail to produce a product, the transformation may not result in any ...
Over the past decades, neuroscientists have made great strides to map brain regions and allocate specific functions to them. Electrical recordings and lesioning studies have been essential for this task. However, most lesioning methods destroy areas larger than intended and may ...
Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV ...
In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research! This ...
Updated April 21, 2020 Picture this: you’re a busy scientist anxiously awaiting the arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last additional experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
