Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory.
Our new AAVs include:
- Control AAVs for serotype testing
- jGCaMP7c for calcium detection
- PSAM4 GlyR for chemogenetic inhibition
- Flp expression for intersectional studies
- And more!
Control AAV
We’re always excited to add new control AAVs to our inventory since we know that scientists need these tools both for pilot experiments and for interpreting the results of studies. When planning AAV experiments, scientists often have to choose the best serotype for their particular study, which includes comparing the transduction patterns of different AAV serotypes. To help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available in various serotypes of two popular control AAV encoding GFP.
Check out our inventory of serotype testing AAV
New control AAVs
| Plasmid | Serotype | Name |
| 105530 | AAV1 | pAAV.CMV.PI.EGFP.WPRE.bGH |
| 37825 | AAV1 | pAAV-CAG-GFP |
| 59462 | AAV2 | pAAV-CAG-tdTomato (codon diversified) |
| 114472 | AAV5, AAV8 | pAAV-hSyn-mCherry |
| 50459 | AAV9, AAVrg | pAAV-hSyn-DIO-mCherry |
| 37825 | AAV1, 2, 5, 8, 9 (20 µL size serotype testing) | pAAV-CAG-GFP |
| 50465 | AAV8, 9 (20 µL size serotype testing) | AAV-hSyn-EGFP |
Biosensor AAV
The ability to detect the neurotransmitters and other bioactive molecules is integral to the study of neuroscience. To this end, new biosensing AAV are always being developed and we are excited to offer these useful tools to the community. Among other new biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018).
See our full biosensor AAV inventory
New glutamate sensors
| Plasmid | Serotype | Name |
| 106176 | AAV1 | pAAV.hSynapsin.SF-iGluSnFR.S72A |
New calcium sensors
| Plasmid | Serotype | Name |
| 104496 | AAV1 | pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE |
| 105322 | AAV1 | pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE |
| 111262 | AAV1 | pAAV-hSynapsin1-axon-GCaMP6s |
| 112005 | AAV9 | pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) |
| 112010 | AAV9 | pAAV-hSynapsin1-FLEx-axon-GCaMP6s |
New voltage sensors
| Plasmid | Serotype | Name |
| 119036 | AAV1 | pAAV-hsyn-flex-Voltron-ST |
DREADDs / Chemogenetics AAV
We have also expanded our inventory of chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited PSAM AAV, which encode engineered ligand-gated ion channels that can be activated with FDA-approved drugs.
See our inventory of DREADDs / Chemogenetics AAVs
New DREADDs
| Plasmid | Serotype | Name |
| 75033 | AAV1 | pAAV CD68-hM4D(Gi)-mCherry |
| 50467 | AAV2 | pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine |
| 121538 | AAV5 | pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) |
| 121539 | AAV5 | pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) |
New chemogenetics AAV
| Plasmid | Serotype | Name |
| 119741 | AAV5 | AAV SYN flex PSAM4 GlyR IRES EGFP |
Recombinase AAV
Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV.
See our recombinase AAV inventory here
New recombinases
| Plasmid | Serotype | Name |
| 55637 | AAVrg | pAAV-EF1a-Flpo |
| 87306 | AAV1 | AAV pEF1a-DIO-FLPo-WPRE-hGHp |
Other new viral vectors
New optogenetics inhibitors
| Plasmid | Serotype | Name |
| 99039 | AAVrg | pAAV-CamKII-ArchT-GFP (PV2527) |
| 105679 | AAV5 | ppAAV-CKIIa-stGtACR1-FusionRed |
New tetracycline transactivators
| Plasmid | Serotype | Name |
| 99120 | AAV1 | pAAV-ihSyn1-tTA |
| 99121 | AAV1 | pAAV-ihSyn1-DIO-tTA |
Viral vectors coming soon!
These vectors listed below should be released at Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be notified when a particular catalog item is available for order please email help@addgene.org with the vector name and serotype you’d like to be notified about.
Controls
| Plasmid | Serotype | Name |
| 37825 | AAVrg (20 µL size serotype testing) | pAAV-CAG-GFP |
| 50465 | AAV1, 2, 5, rg (20 µL size serotype testing) | pAAV-hSyn-EGFP |
Recombinases
| Plasmid | Serotype | Name |
| 55634 | AAV1 | pAAV-EF1a-mCherry-IRES-Flpo |
| 55637 | AAV1 | pAAV-EF1a-Flpo |
Chemogenetics
| Plasmid | Serotype | Name |
| 119472 | AAV5 | AV SYN PSAM4 GlyR IRES EGFP |
Biosensors
| Plasmid | Serotype | Name |
| 105322 | AAV1 | pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE |
| 105714 | AAV8 | pAAV-Ef1a-fDIO-GCaMP6s |
Optogenetics
| Plasmid | Serotype | Name |
| 124651 | AAV9 | pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 |
| 124603 | AAV9 | pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 |
Additional resources on the Addgene blog
- Read all of our viral vectors blog posts
- Find tips for preparing AAVs
- Download our viral vectors 101 eBook
Resources on Addgene.org
- Learn about Addgene's viral service
- Find viral protocols and videos
- Find AAVs for your research
Topics: Viral Vectors, Addgene’s Viral Service
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