Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you have been in the cloning trenches yourself. Modular cloning, or MoClo, is a systematic cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning ...
The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment. With CRISPR entering the clinic for correction of disease-causing alleles, and the growing need for gene editing in research, the old ...
What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21 additions we've made to our viral vectors repository this year (and it's only March!)
At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute them to scientists worldwide. What's more, depositing is free! We've written this post as a step-by-step guide to ...
Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, their role in the immune response, and the different antibody isotypes found in mammals.
Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.
Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the world. In 2004, that was a fairly simple goal… and we had no idea how much our understanding of what accessibility is would change over the next twenty years. But learn we ...
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