Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE isotype?"
In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and exogenously expressed proteins. Here we will review what advantages degron tags can offer over other protein control methods and compare the commonly available types ...
As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you have been in the cloning trenches yourself. Modular cloning, or MoClo, is a systematic cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning ...
The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment. With CRISPR entering the clinic for correction of disease-causing alleles, and the growing need for gene editing in research, the old ...
What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21 additions we've made to our viral vectors repository this year (and it's only March!)
At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute them to scientists worldwide. What's more, depositing is free! We've written this post as a step-by-step guide to ...
Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, their role in the immune response, and the different antibody isotypes found in mammals.
