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Hot Plasmids December 2018 - C. elegans RNAi, TurboID, detecting viral outbreaks, and protein binding specificity tools

Posted by Various Addgenies on Dec 6, 2018 8:34:06 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

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Topics: Hot Plasmids

Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

Posted by Various Addgenies on Nov 27, 2018 9:00:06 AM

By Susanna Bachle and Ina Ersing

Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce the 5 awardees and their research. These researchers span four continents and research diverse topics including microbiology, brain injury, drug discovery, and synthetic biology. Read on to learn more about the five awardees and their promising research projects.

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Topics: Seeding Labs

Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

Posted by Various Addgenies on Sep 18, 2018 9:33:20 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.

Read More >

Topics: Hot Plasmids

Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Posted by Various Addgenies on Jun 27, 2018 9:16:28 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.

Read More >

Topics: Hot Plasmids

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Posted by Various Addgenies on May 15, 2018 8:43:38 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.

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Expanding the Optogenetics Toolbox with CRY2clust

Article contributed by Brook Pyhtila

 Listen to the CRY2clust podcast segment

The Won Do Heo lab at the Korea Advanced Institute of Science and Technology (KAIST) and Institute for Basic Science (IBS) has developed another useful optogenetic tool that enables robust and efficient oligomerization of target proteins in response to blue light. This tool, CRY2clust, was created by adding a 9-residue peptide to the C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a conformational change that permits it to bind to the CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein. After fusing the CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation with blue light.  Similarly, light can also be used to control homo-oligomerization of a protein fused to CRY2, although some studies have shown that this clustering only happens under certain conditions.

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Topics: Hot Plasmids

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