You’ve acquired your long-awaited data – and it looks great! There are some exciting trends in your experimental groups, but how do you determine if they deserve that oh-so coveted asterisk of significance? You’ve already done the heavy lifting, so don’t let the statistical ...
Turn it on? Turn it off? Turn it down, but not forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression control tools as well as ...
Transcription, translation, and prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA – right?
Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway, designing the best donor DNA, and avoiding common mishaps in this class of ...
We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas family of proteins has many useful genome engineering enzymes, each with their own unique features and quirks. Here, we will review the genome engineering ...
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You’ve committed to the daunting task of finding new factors in your biological system of interest, the so-called “fishing expedition.” Your first question is: to screen or to select? Which method will work best for your scientific question? If you’re thinking, ‘wait, those ...
We’ve all heard “Get that tube on ice!!” and “I hope it isn’t degraded” when scientists talk about their precious RNA samples. RNA is inherently less stable than most macromolecules used in scientific research such as DNA or protein. It comes as no surprise then that stability ...
