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Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology lab will undoubtedly be asked to design, modify, or construct a plasmid. A plasmid is a small circular piece of DNA found in bacterial cells, and someone new to plasmids may ...
Plasmid incompatibility is defined as the inability of different plasmids to be maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover why this happens, how it might affect your work, and how understanding it can be used for good. First, why are plasmids ...
As with DNA isolation, scientists commonly rely on RNA isolation kits to make their life easier. Recently, we published a blog on DNA purification without a kit that outlined several reasons why doing something without a kit has advantages: less plastic waste, less expense, and ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification kits have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due to their expense and requirement for ...
There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet alone study.
