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What if after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation? Great news. A subset of our ...
You’ve prepped your DNA and you’re ready to get started on the next step of your experiment. But in many cases, you won’t see any signs of DNA in your final tube after purification. How do you know if you actually have DNA in your tube without seeing it? There are many ways to ...
Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the ...
Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important to consider your experiment. It will dictate the amount of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation in 3 ...
If you use a kit for DNA purification or if you use a DIY purification protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the availability of materials is a core part of our mission. In no situation is this more true than the current global pandemic in which scientists race to find vaccines, ...
