By Michael G. Lemieux
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One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila ...
This post was contributed by guest blogger Meghan Monroy, a graduate student in Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning techniques are ...
Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to ...
This post was contributed by guest blogger, Jessica Sacher, a microbiology PhD student at the University of Alberta studying with the Szymanski lab. Reasons to study how a phage recognizes its host Bacteriophages (viruses that prey on bacteria) may be the most numerous and most ...
Hopefully you know that, if you’re an academic researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections of plasmids as kits with greatly reduced costs per ...
This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated with low morbidity and mild symptoms. This changed in ...
This post was contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis is a highly versatile technique that can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in ...
