By Tyler Ford
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This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated with low morbidity and mild symptoms. This changed in ...
This post was contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis is a highly versatile technique that can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in ...
This post was contributed by guest blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics program and researcher in the Lu Lab at MIT. Note: The following blog post reduces the content of our paper, “Synthetic recombinase-based state machines in living cells” (1), ...
Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from a PCR reaction but not the newly synthesized product from a site-directed mutagenesis experiment? The answer ...
This post was contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins are modified through phosphorylation (1). However, the expansion ...
This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab are replaced by improved ...
This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in ...
