By Susanna Stroik
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We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas family of proteins has many useful genome engineering enzymes, each with their own unique features and quirks. Here, we will review the genome engineering ...
This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA and introduced it into your target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In this blog post we’ll explain ...
This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik. The advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, base ...
Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp new CRISPR material for you! The CRISPR field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook ...
Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems were first discovered in bacteria, most CRISPR-based genome engineering has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double ...
Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific DNA ...
Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple ...
