By Guest Blogger
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If you’re interested in studying immunology or subpopulations of cells, you’ll soon find yourself encountering flow data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. But in order to plan ...
Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."
In a world where so much is out of your hands, it’s helpful to focus on something controllable, like experiments (and their controls!). This blog post will discuss the ins and outs of controls for biological experiments, starting with general controls and then moving on to ...
Fighting with antibodies to produce immunohistochemistry images that are crisp, bright, and lacking in non-specific staining can be a challenge in the best of cases. But it can be particularly challenging when your only antibody option is from the same species as your tissue ...
You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you need are some of your favorite proteins purified to carry out your plan. With a little thoughtful planning, affinity tags can make protein purification a cinch. Types of ...
Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue type, such as PBMCs or tumors, and moreso than other methods, the identification and isolation of rare cell types. In ...
Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously only visible via techniques like super-resolution microscopy or electron microscopy. Since its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in ...
