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When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of certain markers on the surface or the inside of your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV) light, they do so via ...
In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to another. When you label your samples with multiple antibodies, the fluorescent probes on the antibodies may have similar emission spectra, meaning they will emit fluorescent ...
The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise awareness of the economic and cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids too, though not just for ...
When using flow cytometry to analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure your cells of interest. In many experiments you’ll be working with a heterogeneous cell population, for example from a processed piece of ...
Integrins are essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and extracellular environments. Found on nearly every cell in the human body, integrins are key to a diverse array of processes, including cell adhesion, migration, ...
While the antibodies present throughout our bodies carry out plenty of roles just the way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly because, well, antibodies are kind of hard to see. To solve this issue, researchers ...
Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, their role in the immune response, and the different antibody isotypes found in mammals.