Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an RNA-guided nuclease that targets and cuts genomic DNA. The interplay between Cas9 (which causes the breaks) and host cell DNA repair factors ...
When cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable but fleeting. “What’s been shown by others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly targeted therapies,” said ...
This post was contributed by guest blogger Tessa Cressey. The highly pathogenic Ebola virus belongs to the group of nonsegmented negative sense RNA viruses, along with other viruses that cause disease in humans such as measles, mumps, and rabies. Research on Ebola virus has been ...
Have you ever been reading a paper and thought to yourself, “Oh! This plasmid would be perfect for my next experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid Campaign.
The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time to move ahead with your infection and make your stable cell line. While we’ve all experienced the pressure to move a project ...
With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work (H-index for instance), but ...
Whenever possible, we love to give scientists the opportunity to share their knowledge. One of our goals is to be a go-to source of information on recent advances in the biological sciences and techniques that simplify and expedite research. We recognize, however, that we can’t ...
