Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new ...
Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular biology and genetics. It can be used to characterize the function of certain regions in a promoter or gene, as well as to study the effects of inactivating/activating ...
Meetings often get a bad rap as annoying interruptions to our “real” work. However, a well-run meeting can have quite the opposite effect. A great meeting should produce collaboration - a sense of dialogue and community among participants, clarification - new and useful ...
Have you ever found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help make the most of these pages, we've written this post to guide you through them and make the best use of all the information provided by your colleagues.
Updated Mar 26, 2020. At their most basic level, CRISPR/Cas9 genome editing systems use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome and a small RNA (gRNA) to guide this nuclease to a user-defined cut site. After reading this post, we hope you ...
Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting. CRISPR is often used for animal germline modification, to repair or add in disease-causing mutations, but, until recently it hadn’t been used to treat disease postnatally. ...
In the third installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his graduate work in Professor Jim Collins' lab (then at Boston University, now at MIT) where he worked on understanding bacterial antibiotic resistance. During ...
