By Tyler Ford
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The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time to move ahead with your infection and make your stable cell line. While we’ve all experienced the pressure to move a project ...
With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work (H-index for instance), but ...
Whenever possible, we love to give scientists the opportunity to share their knowledge. One of our goals is to be a go-to source of information on recent advances in the biological sciences and techniques that simplify and expedite research. We recognize, however, that we can’t ...
Over the past decade, scientists have developed and fine tuned many different ways to clone DNA fragments which have provided appealing alternatives to restriction enzyme cloning. These newer technologies have become more and more common, and for good reason. They offer many ...
This post was contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and a Postdoc at Indiana University School of Medicine. It’s not always easy to figure out what you want to do after graduate school, at least not while ...
If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions or homology-directed repair (HDR) to create precise genome edits. But have you heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated ...
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new ...
