Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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In July 2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated virus (AAV) to scientists around the world. We began with only a few inventory items offered domestically, but by the end of 2016, we expanded our viral inventory to 25 ...
Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it easier for you to find ...
In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University of Health and Allied Sciences in Ghana. He’s done research at a number of institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of ...
Restriction enzyme cloning is the workhorse of molecular cloning; however, one of its biggest limitations is that sequence modifications can only be made at restriction enzyme cut sites. The lambda red system is an alternative method that can be used for cloning or genome ...
This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that can be used to treat it. For those who are running out of hope with more traditional treatments, such as surgery, radiation, and ...
If you’re into cloning, you’re probably aware that there are several methodologies currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning ...
Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the fraction of that total with the indicated function. One huge reason CRISPR has become such a popular ...
