Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Restriction enzyme cloning is the workhorse of molecular cloning; however, one of its biggest limitations is that sequence modifications can only be made at restriction enzyme cut sites. The lambda red system is an alternative method that can be used for cloning or genome ...
This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that can be used to treat it. For those who are running out of hope with more traditional treatments, such as surgery, radiation, and ...
If you’re into cloning, you’re probably aware that there are several methodologies currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning ...
Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the fraction of that total with the indicated function. One huge reason CRISPR has become such a popular ...
Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again this year!
One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila ...
This post was contributed by guest blogger, Dalila Cunha de Oliveira. Bricking Science is an idea built, literally, 'brick-by-brick' to introduce people all around the world to the lives of researchers and PhD students. Everybody in science knows that there are many ways your ...
