Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Colorful CRISPR technologies are helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent in situ hybridization (FISH) and chromosome ...
When preparing to graduate from university, many students are confronted with the question ‘what now?’ This is often a hard question to answer if you plan on leaving academia, but don’t quite know what you do want to do or even what careers are available to scientists. It is all ...
Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure ...
Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you dread the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you get things moving - remember you're becoming an expert ...
Science rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has made many more Science rap videos to teach students around the ...
High-throughput cloning, in a nutshell, is the systematic combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although the specific sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same ...
When facing a cloning project, scientists are no longer limited to traditional restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given set of resources, time, and experimental needs. Since its invention in the late ...
