Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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If you are interested in using lentiviral vectors to introduce your favourite gene into your favourite cell line or into primary cells, this blog article will give you some tips to plan your experiment using the lentiviral vector system. Viral vectors have been increasingly ...
This is the first half of a two-part interview with Vini Mani and Amy Gilson from Science in the News at Harvard University. Effective science communication is required to promote public support for research and to keep useful discoveries coming. At Addgene, we’re huge ...
This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one of the greatest examples of survival of the fittest. Cancer cells find a way to grow haywire, access and create more vasculature to feed themselves, use the ...
One of the less acknowledged perks of scientific and technical training is that these educational paths prepare you for a vast selection of career options. Scientists are certainly following many diverse career paths these days. A recent National Science Foundation study showed ...
Did you know that one of the quickest ways to submit deposits with more than 10 plasmids is to use our deposit spreadsheet? Our plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material Transfer Agreement (MTA) approval Send ...
We all know that in the lab there are often little tricks that are essential for experiments but that nobody talks about. After months of troubleshooting, those people who did not tell you that essential thing ask incredulously, “You seriously didn’t add 3 microliters of 5 mM ...
Colorful CRISPR technologies are helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent in situ hybridization (FISH) and chromosome ...