Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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One of the less acknowledged perks of scientific and technical training is that these educational paths prepare you for a vast selection of career options. Scientists are certainly following many diverse career paths these days. A recent National Science Foundation study showed ...
Did you know that one of the quickest ways to submit deposits with more than 10 plasmids is to use our deposit spreadsheet? Our plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material Transfer Agreement (MTA) approval Send ...
We all know that in the lab there are often little tricks that are essential for experiments but that nobody talks about. After months of troubleshooting, those people who did not tell you that essential thing ask incredulously, “You seriously didn’t add 3 microliters of 5 mM ...
Colorful CRISPR technologies are helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent in situ hybridization (FISH) and chromosome ...
When preparing to graduate from university, many students are confronted with the question ‘what now?’ This is often a hard question to answer if you plan on leaving academia, but don’t quite know what you do want to do or even what careers are available to scientists. It is all ...
Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure ...
Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you dread the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you get things moving - remember you're becoming an expert ...
