Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression. However, one caveat of FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are ...
This post was contributed by guest blogger, Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science education. Disclaimer: The views presented in this article are those of the author do not represent a formal stance taken by ...
This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first four years (1). In addition to publishing in ...
This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR products are the bread and butter of molecular biology labs the world over. Scientists have traditionally used Sanger sequencing to validate these constructs, as the ...
Rosella is a pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating”) is induced by a lack of ...
What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting and amplifying RNA sequences that could bind very specifically to target molecules. Using a technique called systematic evolution of ligands by ...
Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute for Cell Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge ...
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