Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at least one site. Phosphorylation is particularly important for regulating signal transduction and measuring kinase activity at the single-cell level can aid in drawing ...
Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated ...
This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference series for graduate students. I believe that communication is the single most important skill that scientists need to succeed in their work. While it's not always recognized and ...
This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation, I and other professors at the University ...
This guest post was contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will hopefully help you keep a well organized lab and contamination free environment for successful experiments:
This post was contributed by guest blogger, Tea Pavlek, Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three pillars ...
This post was contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides a solution Proteins - the workhorses of ...
