Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
We're excited to announce that, as in years past, we've been working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases through the BeHEARD Award. Congratulations to Paula Videira, Michael McMurray, and Richard Gronostajski who ...
This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest from the proteome. Rather than ...
Knowing where bacteria are located within their host is often key to understanding their role in both health and disease. To observe bacteria in action, researchers have developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time, ...
Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into the host’s genome. The main challenge with using ...
How many times have you looked at a diagram depicting transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the context of chromatin?” Though it’s true that adding histones and chromatin architecture to ...
The central dogma in molecular biology is DNA→RNA→Protein. To synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most ...
