Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Scientists routinely use techniques to alter gene expression or to label specific cells, but there are too few resources to teach students how to perform these experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology ...
This post was contributed by guest blogger Clare O'Connor an Associate Professor at Boston College. National reports stress the importance of providing authentic research experiences to undergraduate students (1, 2), but educators face significant challenges in designing ...
Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium focused on solutions to reproducibility issues in the biological sciences and featured speakers from academia, industry, nonprofits, and ...
This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent protein database As recognized by the 2008 Nobel Prize, fluorescent proteins (FPs) have become one of the most indispensable tools in ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
This article was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making ...
RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering the genome, but Cas13s have higher on-target specificity. New work from Konermann et al. and Yan et al. describes new Cas13d enzymes that ...
