By Karen Guerin
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This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows washing away of unbound ...
As the saying goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires ...
Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many ...
We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going strong, the AAV service has shown incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV ...
This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For my PhD I studied a synaptic protein that is linked to neurodegeneration. The level of this protein is decreased in Alzheimer’s ...
What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses, a capstone paper I really wanted to hit out of the park, part time work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer ...
Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of ...
