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If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices to make when designing the experiment. What detection method? What membrane? What should you block with?
Now that you know how to read flow plots and have designed your first flow panel, you’ll load your samples into the cytometer and see one of two results for your antibody of interest: two clear populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk ...
What do a viral vector production facility, food allergy testing lab, and the grad student down the hall from you have in common? All of them rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently use qPCR with a standard curve to ...
While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow for ...
I never would have imagined that my summer before high school senior year would have been spent within a lovable community that has allowed me to expand my knowledge more than ever before. Before joining Addgene, I had spent most of my junior year stressed out applying for ...
The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging methods. Across these methods, the following components are needed:
Western blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect more than one protein in your sample? Or what if something weird happened during your western and your ...
