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Switch to GECO? An overview of AAV Encoded Calcium Sensors

Posted by Leila Haery on Apr 26, 2018 9:24:32 AM

As part of our partnership with the Penn Vector Core, we will be expanding our inventory of tools for calcium sensing. In this post, we’ll review the main categories of sensors we’ll have available.

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Topics: Fluorescent Proteins, Viral Vectors

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Posted by Various Addgenies on Apr 23, 2018 10:00:31 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. Below you'll find our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit please sign up here.

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Topics: Hot Plasmids, Podcast

Transferable Skills Guide: External Collaborations

Posted by Susanna Bachle on Apr 19, 2018 9:25:44 AM

A recent survey of PhDs found that many researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where you might need some help. Today in our transferable skills guide: Collaborating with others outside your organization also known as external collaborations.

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Topics: Career, Career Readiness, Transferable Skills Guide

Delivery Methods for Generating iPSCs

Posted by Beth Kenkel on Apr 17, 2018 9:37:57 AM

The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. In that time, scientists have used almost all available approaches for generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. However it’s difficult, especially as a newbie reprogrammer, to decide which method to use. This post provides a brief overview of reprogramming methods with the goal of helping readers choose a strategy suited to their research.

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Topics: Stem Cells

Podcast: Interview with Addgene's Founders

Posted by Tyler Ford on Apr 13, 2018 8:51:50 AM

In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this is the episode for you.

 

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Topics: Inside Addgene, Podcast

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