Plasmids 101: Choosing an Antibiotic Resistance Gene

By Susanna Stroik

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Cartoon showing the different types of controls necessary for a microscopy experiment. Full details are in alt text in post.
A cartoon of the cycle of an adenoviral particle. Beginning at the clathrin-coated pit, infecting virions are endocytosed and imported into the nucleus through steps of capsid disassembly endosomal membrane disruption, and dynein-dependent transport of the viral payload to nuclear pores via microtubules. It is a circular diagram going through time, with arrows between each of the steps. Many of the later steps are not mentioned here, but they regard manufacture of new virions, which does not happen with most viral vectors.
Schematic showing CIB1-fluorescent protein-Rab attached to vesicles and free CRY2 protein. 488 nm light causes CRY2 and CIB1 to bind each other and form a cluster with the Rab protein and vesicles.
Blugene holding a number 2 pencil
Schematic summarizing the problem with species on species immunohistochemistry. The schematic is titled Species on Species Staining - The Problem and shows a cell membrane with a target protein and an endogenous IgG. A primary antibody is bound to the target and secondary antibodies conjugated with fluorophores are bound to both the primary antibody and the endogenous IgG.
Diagram of HIS-tagged protein column purification
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