By Rachel Leeson
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What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20 additions we've made to our viral vectors repository since March.
CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but ...
When it comes to labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated antibodies directly bind to epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs within ...
Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019; Chen & Liu, 2023). This method uses a prime editor (typically ...
All plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or may not be synchronized with the replication of the ...
This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday, June 9th, we hosted our 20th anniversary party for all Addgenies to celebrate together. We were even able to have many of our remote workers travel to Addgene's headquarters in ...
When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples. While you are probably familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as an application. These controls will ...
