Performing In Vivo CRISPR Screens Using the FITS Approach

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 A viral particle enters a host cell, enters the host nucleus, is transcribed into RNA, and is translated into protein.
Addgene CRISPR distribution and deposits. (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year. (D) Cumulative CRISPR pooled libraries shipped by year.
Four schematics of protein labeling strategies with pros and cons list for each.
Graphic showing a narrow range host plasmid with one ORI, and a broad range host plasmid with three ORIs with variable inserts.
a flow plot divided by a quadrant.

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